Cell culture is a process that involves the growth and development of cells outside of a living organism. It provides a controlled and easily manipulated environment for studying cell activity, their response to various stimuli, and the production of recombinant proteins and drugs.

The optimal growth environment for each cell type differs, but typically requires appropriate temperature, pH, osmotic pressure, gas, and nutrient supply. A crucial component of cell culture is the selection of a properly formulated cell medium, which provides the necessary components such as amino acids, minerals, vitamins, and carbohydrates.

In recent years, there has been a focus on developing culture media that can mimic the physiological environment of a given tissue. AFP Pro has been at the forefront of this pursuit, offering both liquid and solid culture media that are versatile for a wide range of applications and precisely tailored to specific cell types. You can trust that all of the media we offer are of the highest quality, manufactured according to the original recipe, and subjected to rigorous quality control tests.

CLASSICAL LIQUID MEDIA

Eagle’s Basal Medium is a synthetic basal medium with a simple composition that is widely used for culturing various mammalian cells. This BME medium fulfills all the necessary requirements for the growth of cells in vitro, including primary mammalian fibroblasts.

Application

  • Cell culture of mammalian eukaryotic cells

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 24 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: BME-A+L
  • Packaging: Available in 500 ml bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

Minimum Essential Medium is widely used in the culture of a wide range of cells. MEM Advachel+ is a medium enriched with Earle salts and L-glutamine. Does not contain hormones, lipids or growth factors, making it usually necessary to add FBS (fetal bovine serum).

Application

  • Cell culture of different types of adherent cells or cells growing in suspension

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: MEM-A+L
  • Packaging: Available in 500 ml bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

DMEM’s Advachel+ High Glucose formulation, based on MEM medium, contains a high concentration of glucose (4.5 g/L) and higher concentrations of ingredients such as minerals and vitamins. The medium’s composition was further enriched with L-glutamine. The medium does not contain lipids, growth hormones, or proteins essential for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of a wide range of mammalian cells

Technical Specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: DMEM-A+HGL
  • Packaging: Available in 500 ml bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

DMEM’s Advachel+ High Glucose and HEPES formulation is based on MEM medium. It contains a high glucose concentration (4.5 g/L), HEPES buffer (25 mM), and higher concentrations of ingredients such as minerals and vitamins. The medium was further enriched with stable glutamine. The medium does not contain lipids, growth hormones, or proteins essential for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of a wide range of mammalian cells

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: DMEM-A+HGHL
  • Packaging: Available in 500 ml bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

DMEM’s Advachel+ Low Glucose formulation, contains a low concentration of glucose (1 g/L) and provides the basic nutrients necessary for cell culture. The composition of the medium has been enriched with L-glutamine and sodium pyruvate. DMEM Advachel+ Low Glucose medium does not contain, lipids, growth hormones or proteins, necessary for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of numerous cell types

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water.

Orders and delivery

  • Product code: DMEM-A+LGL
  • Packaging: Available in 500 ml bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

The RPMI 1640 Advachel+ formulation contains glucose at a concentration of 2g/L, L-glutamine, and phenol red. However, it does not contain lipids, growth hormones, or proteins, which are essential for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of mammalian cell lines

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at 2-8°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: RPMI-A+L
  • Packaging: Available in 500 ml bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

CLASSICAL POWDER MEDIA

Minimum Essential Medium is widely used in the culture of a wide range of cells. MEM Advachel+ is a medium enriched with Earle salts and L-glutamine. Does not contain hormones, lipids or growth factors, making it usually necessary to add FBS (fetal bovine serum).

Application

  • Cell culture of different types of adherent cells or cells growing in suspension

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store at 2-8°C
  • Shelf life: 36 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: MEM-A+P
  • Packaging: Available in 1L, 5L or 10L bottles
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

DMEM’s Advachel+ High Glucose formulation, based on MEM medium, contains a high concentration of glucose (4.5 g/L) and higher concentrations of ingredients such as minerals and vitamins. The medium’s composition was further enriched with L-glutamine. The medium does not contain lipids, growth hormones, or proteins essential for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of a wide range of mammalian cells

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store at 2-8°C
  • Shelf life: 36 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: DMEM-A+HGP
  • Packaging: Available in 1L, 5L or 10L bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

DMEM’s Advachel+ Low Glucose formulation, contains a low concentration of glucose (1 g/L) and provides the basic nutrients necessary for cell culture. The composition of the medium has been enriched with L-glutamine and sodium pyruvate. DMEM Advachel+ Low Glucose medium does not contain, lipids, growth hormones or proteins, necessary for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of numerous cell types

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store at 2-8°C
  • Shelf life: 36 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: DMEM-A+LGP
  • Packaging: Available in 1L, 5L or 10L bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

The RPMI 1640 Advachel+ formulation contains glucose at a concentration of 2g/L, L-glutamine, and phenol red. However, it does not contain lipids, growth hormones, or proteins, which are essential for cell growth. Therefore, it is usually necessary to supplement the medium with FBS.

Application

  • Cell culture of mammalian cell lines

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store at 2-8°C
  • Shelf life: 24 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: RPMI-A+P
  • Packaging: Available in 1L or 10L bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

SPECIAL MEDIA FOR STEM CELL CULTURE

B8 Advachel+ medium is designed for human stem cell culture. It supports the growth and development of human embryonic stem cells and human induced pluripotent stem cells and does not require the addition of FBS.

Application

  • Cell culture of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at -20 – -10°C
  • Shelf life: 12 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: B8-A+L
  • Packaging: Available in 500mL bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

B9 Advachel+ medium is a simple modification of B8 Advachel+ medium created by adding a protein-recombinant albumin. This medium is dedicated to the culture of bovine satellite cells (skeletal muscle stem cells). It does not require supplementation with serum.

Application

  • Cell culture of bovine satellite cells

Technical specifications

  • State of matter: liquid
  • Purity: Sterile product, filtered for sterility
  • Storage: Store at -20 – -10°C
  • Shelf life: 12 months in original packaging

Safety information

  • Classification: The product is not considered hazardous according to CLP Regulation (EC) No 1272/2008
  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water.

Orders and delivery

  • Product code: B9-A+L
  • Packaging: Available in 500mL bottles
  • Transport conditions : Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)
  • Test results (microbiology, endotoxins, mycoplasma)

Customer service department

contact@afp-pro.com

BIOTECHNOLOGICAL YEAST EXTRACTS

Yeast Extract YeastPRO 100 is produced by autolysis of Saccharomyces cerevisiae cultivated under controlled conditions. Serving as a nitrogen source in culture media, it can be widely applied in various areas, including industrial fermentation processes. The extract is rich in B group vitamins.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 24 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: YPRO-100
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Extract YeastPRO 200 is produced by autolysis of Saccharomyces cerevisiae cultivated under controlled conditions. As a nitrogen source in culture media, it finds broad application in the following areas: Cell culture media and microbiological testing; industrial fermentation for the production of amino acids, organic acids, antibiotics, enzymes, vitamins, and other products. . The extract is rich in B group vitamins.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 24 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: YPRO-200
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Extract YeastPRO 300 is produced by autolysis of Saccharomyces cerevisiae, grown under controlled conditions. It serves as a nitrogen source in culture media and is widely used in various applications, including microbiological testing,  industrial fermentation and cell culture media. The extract is naturally rich in B-group vitamins.

Application

  • Cell culture media

Technical specification

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: YPRO-300
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Extract YeastPRO 400 is an Ultra-filtrated yeast extract powder derived from the autolysis of Saccharomyces cerevisiae cultivated under controlled conditions. It is i.a. suitable for  Cell Culture, Biopharmaceuticals, and Diagnostics culture media. The extract is rich in B group vitamins.

Application

  • Cell culture media

Technical specification

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: YPRO-400
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

YEAST NUTRIENTS

Yeast Nutrient NutriUP 100 is composed of yeast extract powder derived from the autolysis of Saccharomyces cerevisiae cultivated under controlled conditions. It functions as a nitrogen source in culture media and has a wide range of applications, including use in industrial fermentation processes. The formula is additionally enriched with high levels of B group vitamins.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: NUP-100
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Nutrient NutriUP 200 is made from yeast extract powder obtained through the autolysis of Saccharomyces cerevisiae, cultivated under carefully controlled conditions. Serving as a nitrogen source in culture media, it is widely utilized in various fields, including cell culture media, microbiological analysis, and industrial fermentation. It plays a key role in the production of amino acids, organic acids, antibiotics, enzymes, vitamins, and other bio-based compounds. The formula is fortified with high concentrations of B-group vitamins to enhance its nutritional value.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: NUP-200
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Nutrient NutriUP 300 consists of extract powder derived from the autolysis of Saccharomyces cerevisiae grown under controlled conditions. It serves as a nitrogen source in culture media and is widely used in cell culture, microbiological testing, and industrial fermentation for producing amino acids, organic acids, antibiotics, enzymes, and vitamins. Rich in high levels of B-group vitamins, it offers enhanced nutritional value.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: NUP-300
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

Yeast Nutrient NutriUP 400 is an ultra-filtrated product consisting of yeast extract powder obtained through the autolysis of Saccharomyces cerevisiae, cultivated under controlled conditions. It is particularly well-suited for use in cell culture, biopharmaceuticals, and diagnostic culture media. The formula is rich in high levels of B-group vitamins.

Application

  • Cell culture media

Technical specifications

  • State of matter: solid (powder)
  • Purity: Non-sterile product, post-dissolution filtration required
  • Storage: Store in a cool, dry place
  • Shelf life: 36 months in original packaging

Safety information

  • Precautions: : Avoid inhalation and skin contact. In case of contact, immediately rinse the area with plenty of water

Orders and delivery

  • Product code: NUP-400
  • Packaging: Available in 20 kg bags
  • Transport conditions: Controlled temperature or room temperature
  • Price: Available upon request
  • Delivery: Expedited shipping options available.

Quality control documents

  • Quality control certificate
  • Material safety data sheet (MSDS)

Customer service department

contact@afp-pro.com

READY-TO-USE VITAMIN AND MINERAL BIOTECHNOLOGY MEDIA

We also develop full vitamin-mineral media for customers’ needs. For more detailed information, please contact us.

Customer service department

contact@afp-pro.com